Single Cell Dna Amplification Kit

The genomeplex single cell whole genome amplification kit wga4 is designed to amplify the genome of a single cell resulting in a million fold amplification yielding microgram quantities of genomic dna.

Single cell dna amplification kit.

Wga is achieved by pcr amplification of the library molecules using universal oligonucleotide primers. The nebnext single cell low input cdna synthesis amplification module or the smart seq v4 ultra low input rna kit for sequencing clontech 634891 were used. Repli g advanced dna single cell kit for whole genome amplification wga from single eukaryotic cells limited samples or purified genomic dna repli g midi kit. The kit includes all reagents required for cell lysis whole genome amplification enzymatic dna fragmentation and pcr free ngs library preparation.

Genomeplex single cell whole genome amplification kit utilizes a proprietary technology based on random fragmentation of genomic dna and conversion of the resulting small fragments to pcr amplifiable library molecules flanked by universal priming sites. Whole genome amplification from a single cell is now possible with our optimized genomeplex single cell whole genome amplification kit wga4. The genomeplex single cell wga kit wga4 containing the wga dna polymerase accurately amplifies plant dna as shown in the two model systems arabidopsis and tobacco. Error bars indicate standard deviation for 8 21 replicates.

Traditional single cell whole genome amplification methods yield insufficient quantities with significant biased representation. At very low levels of input dna successful amplification relies on reagent quality. Cdna libraries were amplified using the number of pcr cycles shown. In addition the mda reaction time has been shortened to 2 hours.